The seq Module

In bioinformatics, biological sequence data occurs in 3 different ways: As a single sequences, lists of sequences and in form of sequence alignments. These 3 ways of looking at sequence data is encoded in the seq module by 3 different classes. The simplest of this classes is the SequenceHandle, representing a single sequence. To represent a list of sequences, the module provides the SequenceList class and alignment-based data is represented by AlignmentHandle. The main difference between the SequenceList and AlignmentHandle classes is, that the sequence list classes are mainly used sequence-wise, while in Alignments one often looks at an aligned column. This results in a drastically different interface, even if both of them work on lists of sequences.

Sequence IO

Sequence IO is dealt-with in the io module. To load single sequences, use ost::io::LoadSequence(), sequence lists use ost::io::LoadSequenceList(), and alignments use ost::io::LoadAlignment().

Converting between alignments and sequence lists

AlignmentHandle::GetSequences() provides read-only access to a sequence-based view. To create an alignment from a list of sequences, use AlignmentFromSequenceList(const SequenceList&). This method will fail when not all of the sequences have the same length. If the sequence lengths differ, they have to be padded accordingly.